The Employment of Real-Time Polymerase Chain Reaction Using Species-Specific Primer Targeting on D-Loop Mitochondria for Identification of Porcine Gelatin in Soft Candy

نویسندگان

چکیده

Analysis of non-halal components, such as pork and porcine gelatin, in food pharmaceutical products is a need for halal authentication study. This research was aimed to develop species-specific primer (SSP) analyze DNA gelatin soft candy using real-time PCR. The SSP designed NCBI Primer-BLAST software. subjected validation by assessing some parameters, including specificity, sensitivity, repeatability test, linearity. results showed that the PCR with targeting on mitochondrial D-loop specifically able identify presence at an optimum annealing temperature 50.5 °C. coefficient variation (CV) analysis Cq 0.53%, efficiency value (E) amplification 100%. Real-time D-LOOP (forward: ACTTCATGGAACTCATGATCCG; reverse ATGTACGTTATGTCCCGTAACC) can also be successfully used identification candy.

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ژورنال

عنوان ژورنال: Indonesian Journal of Chemistry

سال: 2021

ISSN: ['2460-1578', '1411-9420']

DOI: https://doi.org/10.22146/ijc.60413